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M
ATERIAL AND METHODS
 
Laying hens and diets 
The experiment was carried out in poultry farm LLP "Saru Bulak" in Almaty 
region on laying hens breed "Hajseks white". The flock of medium weight laying 
hens in the first half of their production cycle (24 week of age) was randomly 
allocated to five-tier battery hens of 20 laying hens each, under conventional 
conditions of ventilation, temperature (17-19°C) and lighting (16 h light d
-1
). All 
birds had a free access to diets and water and fed with a standard industrial diet of the 
LLP "Saru bulak". The protocol for this study was approved by the local Animal 
Experimentation Ethics Committee. Feed mixtures contained the same components, 
the only difference was that the mixtures designed for the experimental groups were 
supplemented with vermiculite: (A experimental group) a basal diet (BD) without 
vermiculite (V), (B) 97% of BD supplemented 3% V, (C) BD supplemented with 5% 
V, (D) 97% BD+3% V+ fish meal (1% V and 2% FM) and (E) 95% basal diet and 
5% V+ fish meal (1.5% V and 3.5% FM) (Table 1). The feed additives where the 
vermiculite with fishmeal is the concentration, showed good results during storage 
compared with other relations.


77 
Feeding, weight control and maintenance of the hens was according to 
management guide leading for "Hajseks white". The vermiculite used in this study 
was provided by LLP «Kulantau» (Kazakhstan), brand M-150, fraction was 0.5-3.0 
mm. Treatments were assigned randomly and consisted of the incorporation 
vermiculite in commercial ingredients of diets. The vermiculite is characterized by 
high Fe
2
O
3
content (20.59%); SiO
2
(17.8%); K
2
O (8.18%); Al
2
O
3
(7.22%); MgO 
(6.4%); TiO
2
(2.27%); CaO (1.79%) and FeO (0.56%) (data unpublished). The 
approximate dimensions of the crystals used in obtaining the diffraction data were 
0.4×0.2×0.2 mm. 


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