"фараби əлемі" атты халықаралық ғылыми конференция материалдары
THE EFFECT OF WATER-SOLUBLE FRACTION OF OIL ON GROWTH OF THE MARSH
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thesis114326
| THE EFFECT OF WATER-SOLUBLE FRACTION OF OIL ON GROWTH OF THE MARSH
FROG (RANA RIDIBUNDA) IN THE CHRONIC EXPERIMENT Sutuyeva L.R., Tastan D.A., Kakabayev D.Zh., Ondassynova A.S. al-Farabi Kazakh National University, Almaty, Kazakhstan Nazarbayev Intellectual School, Aktau, Kazakhstan s_leila_aktau@mail.ru Intensive increase in oil production in Kazakhstan leads to pollution of air, surface and groundwater, and through them – of soil and vegetation cover with oil and petroleum products. Pollutants enter the body of hydrobionts, and then the body of vertebrates with polluted water through the food chain, causing diseases and their death, thereby reducing the biodiversity of fauna in the oil-producing regions. Tadpoles of frogs are a convenient object for assessing the state of ecosystems that are susceptible to anthropogenic impact. It is known that a number of environmental pollutants, including oil hydrocarbons , can cause morphophysiological disturbances in anuran amphibian larvae, resulting in their death. In this connection, a study of the chronic effect of water-soluble fraction of oil (WSFO) on the tadpoles of the marsh frog (Rana ridibunda) was conducted. The eggs of R. ridibunda artificially obtained using hormonal stimulation were incubated in aerated dechlorinated water at 20±2°C. After reaching Gosner stage 26 tadpoles were divided into 4 groups: control, 0.05 mg/L, 0.5 mg/L, and 1.5 mg/L of WSFO. Each group consisted of 20 L aquaria containing 15 tadpoles. The experiment continued 60 days. Water changes were made every two days, followed by the addition of appropriate concentrations of WSFO. Morphometric parameters (weight, snout-vent length (SVL), full body length), as well as frequency of morphological abnormalities occurrence were studied. No differencies were observed between tadpoles exposed to 0.05 mg/L of WSFO and control group for all endpoints of the study. The weight of the tadpoles exposed to 0.5 mg/L and 1.5 mg/L of WSFO was decreased by 1.4- and 1.7-fold compared to control group, respectively. The SVL and the full body length of the tadpoles of the experimental groups (0.5 mg/L and 1.5 mg/L) were redused 1.3- and 1.7-fold and 1.3- and 1.6-fold compared to control group, respectively. In addition, a dose-dependent increase in the frequency of morphological abnormalities was observed. The most common were scoliosis, abnormal gut coiling, oedema and diamond-shaped body. Thus, it was shown that the chronic exposure to WSFO suppresses growth and causes morphological abnormalities in R. ridibunda tadpoles. Scientific adviser: Doctor of Biological Sciences, Professor Shalakhmetova Т.М. жүктеу/скачать 2,79 Mb. Достарыңызбен бөлісу:
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