К Л И Н И Ч Е С К А Я М Е Д И Ц И Н А

Document Outline

• 3. M. Honma, A. Kitazawa, A. Cayley, R. V. Williamset all. Improvement of quantitative structure–activity relationship (QSAR) tools for predicting Ames mutagenicity: outcomes of the Ames/QSAR International Challenge Project. Mutagenesis. 2019 Mar; 34(...
• 4. Г.Габдуллина Остеопороз и правильное питание/2018г.
  • BIOETHANOL EMISSIONS
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• Introduction
• Inhibitors of the mammalian target of rapamycin (mTOR) pathway are recognized as perspective antiepileptic compounds (19,21). Such pharmacons are different from classical antiepileptic drugs and cause a broad spectrum of effects, including modificatio...
• Such data favors the mTOR inhibitors as a new and promising approach to epilepsy treatment (4, 8, 9, 11, 15, 18). Antiseizure action of rapamycin – well-known mTOR inhibitor has been shown on kainic acid induced continuous seizures (21), epileptic sta...
• That is why this work's main aim was to investigate the effects of rapamycin upon pentylenetetrazol (PTZ)-induced kindled convulsions. Considering that PTZ-kindled seizures are highly sensitive to valproic acid's antiseizure action [10], the compariso...
• Materials and Methods
• Experiments were performed on male Wistar rats with initial bodyweight 200-250 g. Animals were kept in standard conditions (constant temperature 23o C and relative humidity 60%, 12 hrs dark/light cycles, standard diet, and tap water were given ad libi...
• Kindled convulsions were induced, as described previously [6]. PTZ ("Sigma Aldrich") was given intraperitoneally (i.p.) daily in a dose of 30.0 mg/kg for 21 days. The severity of convulsions was evaluated according to the following criteria:
• Results
• Behavioral characteristics of the convulsions in kindled rats
• Repeated i.p. administration of PTZ (35.0 mg/kg) resulted in the progressive development of seizure manifestations, starting from the third to sixth injection. At the moment of completion of kindling – after the 21-t PTZ administration, a prevalent nu...
• Effects of rapamycin and valproic acid
• Administration of rapamycin in doses of 0.3; 1.0 and 3.0 mg/kg, i.p. resulted in a dose-dependent decrease in the severity of kindled convulsions. Statistical significance was achieved at a dosage of 1.0 mg/kg (Table 1), at which dose 6 of 9 animals w...
• Administration of valproic acid to kindled animals (50.0; 100.0 and 250.0 mg/kg, i.p.) resulted in a dose-dependent seizure-protecting effect that was statistically significant for two doses, 100.0 and 250.0 mg/kg (Table 1) as compared with controls (...
• Table 1
• Statistics performed by Kruscall-Wallis and Mann-Whitney U tests.
• References
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• 6. Chubach V.S., Muratova T.N., Myronenko S.I., Godlevsky L.S. Antiepileptic effects of axitinib on pentylenetetrazol- induced kindling in rats. Epilepsia. 2015; 56 (suppl.1): 0142.
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• Аннотация
• ABSTRACT
• RAPAMYCIN ACTION UPON KINDLED SEIZURE ACTIVITY IN RATS