"фараби əлемі" атты халықаралық ғылыми конференция материалдары
PRODUCTION OF A COMPOSITE MATERIAL BASED ON THE BC FILM WITH
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| PRODUCTION OF A COMPOSITE MATERIAL BASED ON THE BC FILM WITH
IMMOBILIZED B. SUBTILIS P-2 CELLS Maulenbay A.D., Kuli Zh.T., Talipova A.B. al-Farabi Kazakh National University maulenbay.id@gmail.com Successful immobilization of bacteria and enzymes on the BC with the preservation and even increasing in their physiological activity served as the basis for the present study. According to the obtained data, the B. subtilis P-2 strain is best suited for this purpose. These types of activity of the strain can impart future composites both to the properties of the antibiotic and to the properties of the enzyme preparation. There are two main methodological methods for including additional components in the BC. In one of them, the reinforcing material added to the BC during its synthesis; thereby this inserted component incorporated into the film and then becomes part of the polymer structure. However, since the B. subtilis P-2 strain has antibacterial and proteolytic activity, expected that it inhibit the development of the BC producer strain. Therefore, another methodical technique used, namely, the inclusion of cells B. subtilis P-2 carried out by their joint aggregation with an already finished BC film. The nature of changes in the sorption capacity values indicate a gradual increase in the biomass of B.subtilis P-2 cells on the gel film of the BC already starting from the 2-hour contact, which leads to a decrease in the number of suspended cells. By the 24 hours of the experiment, the optical density of the suspension has reached the plateau, and no further reduction occurs. Saturation of the BC of the film with cells of B. subtilis P-2 occurs. The subsequent increase in the contact time of the suspension of bacteria with the carrier up to 96 hours did not lead to an increase in the number of attached cells. Based on the data obtained, concluded that 24 hours is the optimal time for immobilization of B. subtilis P-2 cells on the gel film of the BC under the conditions of our experiment. Scientific adviser: Doctor of biological sciences Savitskaya I.S. жүктеу/скачать 2,79 Mb. Достарыңызбен бөлісу:
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