Оригинальные исследования Лазерная медицина. 2019. Т. 23, вып

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Контакты: Макаров Максим Сергеевич, e-mail: mcsimmc@yandex.ru MORPHOFUNCTIONAL PROPERTIES OF HUMAN PLATELETS AFTER LASER IRRADIATION in situ

Для цитирования:
Макаров М.С., Хватов В.Б. Морфофункциональные свойства тромбоцитов человека, подверженных
in situ
низкоинтенсивному лазерному облучению // Лазерная медицина. – 2019. – Т. 23. – Вып. 2. – С. 26–31.
Контакты:
Макаров Максим Сергеевич, e-mail: mcsimmc@yandex.ru
MORPHOFUNCTIONAL PROPERTIES OF HUMAN PLATELETS
AFTER LASER IRRADIATION
in situ
Makarov M.S., Khvatov V.B.
Sklifosovsky Research Institute of Emergency Care, Moscow, Russia
Abstract
Purpose.
To study morphofunctional characteristics of human platelets irradiated
in situ
with modulated light of the visible spectrum.
Material and methods
.
Donor platelets were harvested by the automatic apheresis, stained with vital dye and irradiated with a laser system
attached to the confocal microscope «Nikon D-Eclipse C1» (Nikon, Japan). Modulated light with long UV-wave (λ = 408 nm), blue light
(λ = 488 nm), green light (λ = 543 nm) and red light (λ = 637 nm) was used for the irradiation; irradiation sessions lasted for 0.5–60 min.
Results.
Аfter low-dose light irradiation with λ = 488 nm and λ = 543, human platelets did not have any visible changes; after irradiation
with red light (λ = 637 nm), platelets with normal biological status were quickly degranulated and activated; degranulation intensity
depended on the irradiation time. A similar effect was registered during long wave UV-irradiation (λ = 408 nm), while the followed platelet
activation was lower than after red light irradiation. Granules offset to the cytoplasm periphery started in 0.5–1 min after red irradiation

27
Оригинальные исследования
Лазерная медицина. – 2019. – Т. 23, вып. 2
and in 10 minutes after ultraviolet one. Total activation of biologically high-grade platelets was seen in 30 and 60 min, respectively.
Conclusion.
Еxposure of human platelets to red and ultraviolet light
in situ
stimulates their spontaneous activation without any damage
to the platelet structure.

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