Communication of the research with scientific research programs. The work
was performeds part of the research project by the budget program 217 "Development
of
science",
with
priority:
Sustainableuse
of
natural
resources,
raw
materialsprocessing and production on the topic: 0176 / GF4 "Environmentalimpacts
of
rocket-carrierlaunching
of
“Proton-M”
in
animal
breeding."
Results of the research
1. Determined 9 operatingwintering with the totalnumber of animals - 2002
heads and 5 settlements - only 48,691 heads are adjacent to the area of the №15
"Proton-M" fall.
2. 3 cards were developed: generals cheme of arrangement areas falling number
15, 25; wintering location map adjacent to the areasof the "Proton-M" fall; zoning
map
related
to
danger:
dangerous
and
red,
and
relativeriskzone.
3. In the dangerous and red zonethere are no agriculturalgroups, at risk - 4, in the
comparativeareas
-
6
wintering
and
4
settlements.
4. Animal products (milk, meat) from affectedareasshowedsignificantchangesin
organolepticcharacteristics, color, smell, physical and chemicalparameters - fat
content,
drymatter,
pH,
lactose,
VFA,
than
in
otherareas.
5. Nutritionalvalue of milk in winteringareas from riskzoneswasreduced by:
aminoacidconcentration (from 4.7% to 26%, the amount of aminoacids - by 12.2%,
ratio
of
the
amount
of
essentialaminoacids
to
non-essential
-
0.89,
aminoacidsscorewasratherminimal for methionine- 68% phenylalanine - 85.4%. );
concentration of the vitamin content (vitamin B1 by 21.25%, vitamin B2 -by 38,6%;
vitamin B12 by 47.3%; B6 - by 48.4%, vitamin C - by 40.3%); concentration of
macro-minerals (magnesium- by 17.2% zinc - by 22.3%, copper - by 26 %, iron - by
37.4%, marganese by 35%); concentration of saturated fatty acids (myristic - by
31.2%,
palmitin-by
38,5%,
stearic-
by
45.2%);
6. Nutritionalvalue of meat from the winteringareas of riskwasreduced by:
concentration of aminoacids (from 3.7% to 25.9%, amount of aminoacids - by 10.7%,
ratio
of
the
amount
of
essentialaminoacids
to
non-essential
-
0.89,
aminoacidscorewasminimal for valine-85.5%, 90.1% for isoleucine, leucine-95.5%,
methionine-65%; 92.25% for threonine, triptophan- 90.7%, 39.6%-phenylalanine ;
concentration of vitamincomposition (vitamin B1 - 31.3%, vitamin B12- 47.3%),
vitamin B6 - 49.4%, vitamin B2 - 52%); concentration of macro-minerals
(magnesium- by 17.3%; sodium - by 34.6%, iron - by 37.4%); concentration of
saturated fatty acids (stearic - 5.5%, palmitin-5,8 %, myristic - 36.4%,
polyunsaturated acid: 12.5% by linoleic, 28.6% by linolenic);
7. In the experimentaltoxicosis 1,1-dimethylaccumulationoccurs in all organs
and tissues of laboratoryanimals. Toxins content exceeds the maximum permissible
concentration and varies from 2,07 ± 0,71 mg / kg to 0,1 ± 0,05 mg / kg.
8. The accumulation of toxins in the milk of cow schangedabruptly, there was a
picture
of
gradual
cumulative
concentration.
9. It is found that after 12 and 24 hours in 1,1-dimethylhydrazinewasdetected in
kidney 0.25 ± 0.07 mg / kg, 0.61 ± 0.18 mg / kg, after 48 hours in liverconcentration
133,81 ± 40,14 mg / kg, and lungs 82,48 ± 24,74 mg / kg. After 72 hours, the
concentration of 1,1-dimethylhydrazine in liverdecreased to 98.62 ± 29.59 mg / kg.
10. It is found that 1,1- dimethylhydrazine is excreted from cow safter 48-72
hours.
11.Livestock products (milk, meat) with experimental1,1-dimethylhydrazinet
oxemia showed significant changes in organoleptic - taste, smell and texture, physical
and chemical parameters - fat content, dry matters, pH, lactose, VFA
12.Nutritionalvalue
of
milk
in
an
experimental
1,-
dimethylhydrazinetoxicosiswasreduced by: aminoacidconcentration (from 3.5% to
25.7%; amount of aminoacids - by 7%, ratio of the amount of essential aminoacids to
non-essential- 0.78, aminoacidscorewas at minimum of 65.1%-methionine,
phenylalanine-85.2%); concentration of the vitamin content (vitamin B12 -15.5%,
vitamin B1 - 28.4%, vitamin B2 - 33.4%, vitamin B6 - 61.5%, vitamin C - 28.5%);
concentration of macro minerals (zinc - 22.3%,copper-30,9%, iron - 36.1%
manganese - up to 85% molybdenum- 82%) concentration of saturated fatty acids
(palmitic
-
27%,
stearin
acid-
33.8%,
myristicacid
-
37%).
13.
Nutritionalvalue
of
rabbit
meat
in
the
experimental1,1-
dimethylhydrazinetoxicosiswasreduced by: concentration ofaminoacids (from 1.2% to
9.1%, amount of aminoacids - by 7%, the ratio of the amount of essentialaminoacids
to non-essential - 0.78, the aminoacidscorewasminimal for methionine - 65.1%,
phenylalanine - 85.3%; concentration of the vitamin content (vitamin B2 - 11.2%
vitamin B1 - by 20.9%, vitamin B12 -by 34.6%, vitamin C - 40.3% ,vitamin B6 - by
60.5%); macro-concentration of traceelements (zinc-17.4%; potassium- 20.6%
manganese -30.8%); magnesium- 40.8%; phosphorus - 44.8%; calcium-45,7%, iron
was 73.5%; 74.5% of molybdenum, sodium - 78.1%); concentration of saturated fatty
acids (palmitin- 17.1%), myristic - 21.5%, stearic - by 25.6%; polyunsaturatedacids
(linoleic 22.3% and linolenoic-34.5%)
Scientific and practical significance. Obtained results were incorporated into
the learning process for preparation of veterinary professionals in higher education
institutes. The research results can be used in the laboratory of veterinary-sanitary
markets examinations, food laboratories carrying out quality and safety control of
animal products, institutions of scientific and educational directions in RSE SIC
"Gharysh- Ecology" and "Gharysh-Ecology" analytical laboratory.
Based on these studies, there are several steps we recommend for practice: in
the preparation of pastures and hayfields in Ulytau district 3 maps with respect to
zoning that we have developed must be used; according to the map on zoning areas
adjacent to the areas of №15 fallbacks fences on the established contact zones should
be set; special signs indicating forbidden characters of animal grazing and hay should
be installed; explanatory work with population living in these areas should be carried.
The need to hold regular organoleptic and physicochemical studies of animal
products of the above areas was confirmed; after each "Proton-M" flight the period of
the use of animal products should be mentioned: for milk - 48 hours, 72 hours for
meat; raise the question of 1,1-dimethylhydrazine inclusion in the registry study to
determine residual amount in meat and milk in the Analytical Laboratory "Gharysh-
Ecology" Zhezkazgan city. Medical and veterinary services should take into account
the fact that animal products from farms adjacent to the areas of fallbacks have low
nutritional usefulness.
Work evaluation.The results of scientific and experimental studies presented
at international scientific conferences:
- "Seyfullinskie readings - 10 "New perspectives of preparation of competitive
personnel and the role of science in the formation of industrial and innovation policy
of the country", dedicated to the 120th anniversary of the birth of S.Seifullin, Astana -
2014g.Vol1, p.91;
- "Seyfullinskie readings - 11" Astana, 2015 .;
-45-Anniversary of the GNU VNIVIPFiT "PROBLEMS AND WAYS OF
HIGH-TECHNOLOGY STOCK BREEDING DEVELOPMENT" Voronezh, Russia,
2015 g.60;
- "New trends in the development of agricultural sciences", Rostov-on-Don,
Russia, 2016 p.19;
- "Veterinary Medicine in the XXI century: problems, methods, solutions,"
dedicated to the 100th anniversary of Veterinary Helminthology in Kazakhstan
foundation, doctors of veterinary sciences, professors NT Kadyrov KATU after
S.Seifullin, Astana.2016
The research results are introduced in educational process of the Kazakh Agro-
Technical University named after S.Seifullin; State University after Shakarim. For
laboratory specialists of veterinary-sanitary examination in Karaganda region,
specialists of research institutions, veterinarians and students of the Faculty of
advanced training as well as students, undergraduates and doctoral students of
veterinary specialties. For research works in the study of this issue in the RSE SIC
"Gharysh-Ecology" and the analytical laboratory "Gharysh-Ecology".
Publication of research results.Main results of the dissertation were published
in 9 publications 3 of which have been published in journals recommended by the
Committee of the MES, the article RJPBBP-RJPBCS - Research Journal of
Pharmaceutical, Biological and Chemical May - June, 2016 RJPBCS, №7 (3)
included in the base of Scopus data, 4 - in the materials of international conferences, 1
in the national conference and one recommendation.
Structure and volume of the dissertation. Dissertation is stated on 192
pages, consists literature review, research sections, which reveal the materials and
methods,
results
of
the
research,
discussion,
conclusions
and
practicalrecommendations. List of references consists of 177 sources. Thesis is
illustrated with 38 tables, 54 figures and supplemented by 7 applications.
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