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Table 4.
Dietary mineral plus protein sources had a significant effect on the FA profile 
in yolk (Table 4). Feeding with supplementing diet led to an increase in total FA 
content, a little changing of level of saturated fatty acids (SFA), increasing dietary 
mono (MUFA) and polyunsaturated fatty acids (PUFA). Regardless of V+FM 
inclusion levels or combination, contributed to a significant increase in the 
concentrations of C18:2 n-6 and C18:3 n-3 fatty acids and total PUFAs in yolk lipids. 
The SFA: palmitic, arachidic acid decreased about 0.1-0.3% while margaric, 
stearic, docosanoic increased about 0.2×0.5% in group E. Addition of vermiculite 
increased clearly the level of myristic (C14:0) acid in yolk eggs in group B (0.54%).
A slight increase of stearic acid was observed when 5% V was added. No significant 
difference in palmitic acid content was observed compared with control. When 3% 
and 5% of V+FM were added, the percentage of 18:1 oleic acid increased (near two 
times in group D and E). There is no significant increase of 16:1 palmitoleic acid 
percentage. Similar results were reported by C
RESPO AND
E
STEVE
-G
ARCI
´
A
(2001). 
Our data shows that the addition of a natural mineral with fish meal significantly 
increased in yolk eggs the level of total n-3 fatty acids (Table 4), with this response 
being primarily related to higher levels of linolenic acid. Similar results were also 


81 
reported by S
OUZA
et al (2008) using fed diets containing microencapsulated fish oil. 
Fish oil is one of the best known sources of n-3 PUFA, as it is rich in EPA and DHA 
(M
ACLEAN
et al. 2006). Especially, it is important to note that PUFAs (linoleic acid 
and alpha-linolenic acid) have displayed protection against lipid per oxidation 
increasing the levels of several cellular antioxidants such as ascorbic acid, α-
tocopherol (C
ACHALDORA
et al. 2008). The linoleic acid significantly increased over 
tree and four times ore in group C, D and E than in control and B group, and gamma-
linolenic seven times higher in group E, which represented a great contribution to the 
sum of PUFA. Most n-6 polygene fatty acids were noted in the yolk eggs hens fed 
with the V+FM feed. However, this content was higher in all the experimental groups 
in comparison to the control group. The results of current study indicated that the FA 
profile in yolk eggs is customized by a diet with mineral and fishmeal. Feeding with 
different kinds of diets containing 3% and 5% ratios of vermiculite and fishmeal can 
be possible to apply in future which was proven by these results including the egg’s 
yolk level of specific fatty acid or mixture of fatty acids thought to be beneficial to 
human health (e.g. oleic acid). 


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