Атты III халықаралық ғылыми-тәжірибелік конференция жинағЫ
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III TOM
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- Results and discussion.
| Materials and methods. The plant sample, A.asiatica was collected from mountain
slopes of the Kaskasu village and air dried at room temperature. The dried material was homogenized to obtain a coarse powder and stored in air-tight bottles. The sample preparation includes successive extraction of plant material with non-polar and polar solvents. About 300 gm of the powdered plant raw material was subjected into successive extraction in different extracts like chloroform, ethanol (96%) and these extracts employed in GC-MS analysis. The Gas Chromatography-Mass Spectrometry (GC-MS) analysis of extracts of A.asiatica were performed using a GC-MS (Model; 6890N/5973N) equipped with a DB-WAXetr column of 30 m length, diameter 0.25 µm film thickness. The column over temperature was programmed from 40 o C to 260 o C for 77 min. The mass range from 34-750 m/z was scanned. 1.0 µL of the extracts of A.asiatica was injected to the GC-MS manually for analysis. Total running time of GC-MS is 77 min. The spectrum of the unknown component was compared with the spectrum of the known components stored in the NIST library. Results and discussion. The results pertaining to GC-MS analysis led to the identification of number of compounds from the extracts of A.asiatica. These compounds were "SCIENCE AND EDUCATION IN THE MODERN WORLD: CHALLENGES OF THE XXI CENTURY" NUR-SULTAN, KAZAKHSTAN, JULY 2019 354 identified through mass spectrometry attached with GC. The sample of chloroform extract was run for 40 minutes. The spectrum profile of GC-MS confirmed the presence of 18 phytochemical components (Table 1) from A.asiatica. The phytoconstituents identified were mainly sterols, terpenoids and alkanes (Fig. 1). Figure 1. GC-MS Chromatogram of chloroform extract of A.asiatica The bioactive components with their retention time (RT), CAS number and area (%) in the chloroform extract of A.asiatica are presented in (Table 1). S. N o Retention time Name of the compounds САS № Peak Area % 1 14.387 m-Xylene 108-38-3 59362 2 14.504 p-Xylene 106-42-3 54279 3 15.058 Cumene 98-82-8 8809 4 15.352 m-Xylene 108-38-3 59362 6 18.320 4-Hydroxy-4-methyl-2-pentanone 123-42-2 175119 7 21.490 Butane acid 107-92-6 5066 8 22.019 β-Malein 489-29-2 3151 9 22.901 β-Selinen 28624-23-9 7853 1 0 23.879 α – Panasinen 56633-28-4 8024 1 1 24.878 Neophytodiene 504-96-1 42438 1 2 25.274 Octadecamethylcyclononasiloxane 556-71-8 4938081 1 3 25.558 Trans-phytol 150-86-7 25607 1 4 29.096 Hexadecanoic acid, ethyl ester 628-97-7 19610 1 5 30.148 Retinol 68-26-8 7043 1 6 30.520 Eicosomethyl cyclodexadasiloxane 18772-36-6 12386 1 7 36.938 Phytol 150-86-7 9159 1 8 39.610 Heptacosane 593-49-7 25389 Table 1. The bioactive components with their retention time |