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RESEARCH GRAIN PROTEIN CONTENT OF THE MUTANT SPRING WHEAT
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thesis114326
| RESEARCH GRAIN PROTEIN CONTENT OF THE MUTANT SPRING WHEAT
Wang Yarong al-Farabi Kazakh National University yarongwang330@gmail.com Bread wheat is a main crop with global importance for food safety and is one of major cereal source of nutrients for both human and animal. Mutagenesis is a powerful tool for generation of genetic variability and therefore crop improvement. Over 80 years this technique was applied for development of new mutant varieties of both seeds and vegetatively-propagated crops. According to FAO/IAEA Mutant Variety Database in 2014 in world were 3220 mutant plant varieties of 214 plant species. Application of mutagenesis in crop breeding has two main steps: selection of individual mutants with improved traits after mutagenic treatment of plant materials and utilization of selected plants or lines in breeding programs. Grain protein content (GPC) is an economically important trait which plays one of the key roles in determination of nutrition quality of wheat grain and also has strong impact on the bread-making and end-use quality of common wheat varieties. Despite of grait importance of GPC advancement in common wheat breeding for high GPC is quite poor. Seeds of spring bread wheat awn-less variety “Almaken” were irradiated with 100 Gy dose from a 60 Co source at the Kazakh Nuclear Centre. Seeds were planted after irradiation to raise M1 plants. M1 generation was grown in experimental field of Kazakh Institute of Agricultural and Farming. Single spikes were harvested from each plant for M2 generation, and selection of best lines based on yield of individual plants continued to M7 genertion. Seeds of best mutants were selected individually in each generation. After harvesting M7 plants, 5 lines were from the original 100 Gy radiation dose were selected. These lines were numbered as follows: 91(89-5),72(82-4),71(79-1),80(82-5),77(79-2) grain protein content was estimated with near-inrared reflectance spectroscopy of whole grains (ZX50 Portable Grain Analyzer) using respective calibration software provided by Zeltex. Three repetitions were studied using 25 grains per 184 line. Almaken dose 100 Gy mutant lines GPC mean 14.34±0.12 and cv.Almaken. GPC varites had 12.78±0.05. 100 Gy mutant lines GPC high 12% when comparing to parent cv.Almaken. Scientific adviser: Doctor of Biological Sciences, Prof. Saule Kenzhebayeva жүктеу/скачать 2,79 Mb. Достарыңызбен бөлісу:
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