"фараби əлемі" атты халықаралық ғылыми конференция материалдары
PREPARATION OF SUSPENSION CULTURE OF PISTIA STRATIOTES
жүктеу/скачать 2,79 Mb. Pdf көрінісі
|
| PREPARATION OF SUSPENSION CULTURE OF PISTIA STRATIOTES
Sharipbay A.A, Amangeldinova M.Е, Tolen G.B. al-Farabi Kazakh National University sharipbayasem@gmail.com It is known that at high concentrations of microelements in the medium, an irreversible violation of the structural components of the cell is observed, while in small amounts, apparently, repair processes occur. In order to obtain the cells of the suspension culture of Pistia Pistia stratiotes, we performed the following experiments with modified Murashige and Skoog medium. Suspension culture of Pistia Pistia stratiotes was obtained from calluses induced from stem segments planted on the modified Murashige-Skoog (MS) medium, which includes some additions to the main components (mg / ml). The culture was grown in Erlenmeyer flasks on a shaker at a temperature of 26 ° C. Subculturing was carried out every 3 days. An intensively growing culture consisting of 90% meristematic cells was used for the experiment. Phytohormones №1 and №2 were added to the nutrient medium. The initial density of the inoculum in suspension cultures is 2.5-3.0.104 cells / ml. The cultivation time is 96 hours. The density of the suspension was determined by direct counting in a Fuchs-Rosenthal chamber in 6 replicates. Concentration was expressed by the number of cells in 1 milliliter. The purpose of the research: to obtain in vitro suspension culture of Pistia Pistia stratiotes. The object and methods of the research: Water plant Pistia Pistia stratiotes, laminar box, Fuchs- Rosenthal chamber. The results of the research: In the culture medium No. 1, the division of cells is inhibited. In the subsequent there was a decrease in the concentration of cells. With the increase in the duration of cultivation and the change in phytohormones in the medium, the lysis of the cells is intensified. So, only with the addition of the modified medium No. 2, growth processes in the cell culture of Pistia Pistia stratiotes are not inhibited. Scientific adviser: Candidate of Biological Sciences, Assoc. Prof. Ernazarova G.I. жүктеу/скачать 2,79 Mb. Достарыңызбен бөлісу:
|