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OH, MY GUT! THE INTERACTION OF miRNAs WITH mRNAs OF COLON CANCER GENES
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| OH, MY GUT! THE INTERACTION OF miRNAs WITH mRNAs OF COLON CANCER GENES
Akimniyazova A.N., Aisina D.E., Talipova A.B., Kuli Zh.T. Al-Farabi Kazakh National University aigul.21@mail.ru Understanding of genetic events driving the pathogenesis of colon cancer is of critical importance to devise new strategies to treat this malignancy. In recent years actively studied the interaction of miRNA with mRNA of genes, responsible for the development of cancer. miRNA play an important role in carcinogenesis. Definition of specific miRNAs and their target genes, participating in carcinogenesis allows us to better understand the mechanism of their regulation. Searching of miRNA's target genes was performed by MirTarget software, created in our laboratory. This software defines beginning of miRNA and mRNA binding sites; localization of binding sites in 5'UTR, CDS, 3'UTR; free energy of hybridization and scheme of miRNA-mRNA nucleotides interaction. For analysis it was selected 157 genes from Genbank (http://www.ncbi.nlm.nih.gov/), most frequently involved in the development of colon cancer. Identified mRNA of AATK, HDAC4, KDM1A, NFE2L2 and RXRA genes, having fully complementary binding sites with miRNA. The mRNA of AATK gene has two binding sites of miR-17-39023-3p in CDS with energy range from -134 kJ/mole to -142 kJ/mole and value ΔG/ΔGm from 94% to 100%. The mRNA of NFE2L2 has multiple binding sites of miR-1-155-3p, miR-11-28656-5p, miR-19-21199-3p and miR-2-3313- 3p located consistently through one nucleotide in 5'UTR, with ΔG value from -115 kJ/mole to -144 kJ/mole. PRKG1 gene has the largest number of clustered binding sites (83), with ΔG value varying from -102 kJ/mole to -149 kJ/mole at ΔG/ΔGm value from 87% to 95%. miR-15-36862-3p and miR-10-29282-3p have 23 homological binding sites in 3'UTR of UMPS gene, located consistently through two nucleotides, with ΔG value from -104 kJ/mole to -108 kJ/mole. The presence of such multiple binding sites indicates strong dependence of gene expression from each of these miRNAs. PLEC gene has thirty three sites, with ΔG value from -108 kJ/mole to -132 kJ/mole. The obtained 181 results show interaction of considered mRNAs with miRNA for statement, that this miRNA could suppress an expression of these genes with increased presumption. Scientific adviser: doctor of biological science, Professor Ivashchenko A.T. жүктеу/скачать 2,79 Mb. Достарыңызбен бөлісу:
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