Code of module: SBB 8
Name of module: Scientific foundations of biology
Name of the discipline: Genetic engineering
Prerequisites: Biochemistry
Post Requisites: Writing and defending a thesis (project) or passing a comprehensive exam.
Рurpose: To introduce various methods of recombinant DNA technology, the main genetic vectors, methods of genetic transformation of pro- and eukaryotic cells, screening of cloned genes, as well as structures and mechanisms of expression of genetic material of pro - and eukaryotic cells, modern methods of genetic engineering, biotechnology.
Brief description: Studies a set of techniques, methods and technologies for obtaining recombinant RNA and DNA, isolation of genes from the body (cells), the impact on them, turning them into other organisms and growing artificial organisms after removal of selected genes from DNA.
Learning outcomes: Fundamentals of fundamental sciences based on the principles of recombinant DNA technology, genetic engineering;
Creation of recombinant DNA, methods of transporting recombinant DNA into cells of bacteria, yeast, insects, animals and plants using vectors (plasmids, viruses, phagemids, etc.), methods of expression and control of transgenes;
Molecular genetic methods: PCR, RT-PCR, restriction, ligation, transformation, transfection, hybridization methods;
he is able to assess the importance of modern methods of genetic engineering, protein engineering, engineering enzymology, cell engineering.
Formed competencies: Through the acquisition of knowledge on the basic genetic and cellular mechanisms of the body's activity, theories of hereditary material at the molecular level, after the course, the competence of the educational direction, conducting research work is formed.
