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9 УЛЬТРАЗВУКОВОЕ ЭКСТРАГИРОВАНИЕ БИОЛОГИЧЕСКИ АКТИВНЫХ
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Для цитирования: Жумагалиева Ш.Н., Аманжолқызы А., Султанова Н.А., Абилов Ж.А. Ультразвуковое экс- трагирование биологически активных веществ Tamarix hispida Willd. // Химия растительного сырья. 2021. №3. С. 283–289. DOI: 10.14258/jcprm.2021038695. Zhumagaliyeva Sh.N. * , Amanzholkyzy A., Sultanova N.A., Abilov Zh.A. USE OF ULTRASOUND FOR EXTRACTION OF BIOLOGICALLY ACTIVE SUBSTANCES FROM TAMARIX HISPIDA WILLD. Al-Farabi Kazakh National University, av. Al-Farabi, 71, Almaty, 050040 (Kazakhstan), e-mail: shynarnur@mail.ru The extractions of biologically active substances from aerial part of Tamarix hispida Willd. over ultrasound have been studied. The results showed that the output of extractive substances using ultrasound was increased to 37.34% under the following conditions: 10% ethyl alcohol in a raw-extractant ratio (1 : 6) for 25 minutes at a 20–25 °С temperature and an ultrasound fre- quency of 35 kHz. The extraction was compared with maceration by yield and chemical composition. As a result of optimization, the output of components from the raw material is increased by 3–4 times at ultrasound frequency from 20 to 35 kHz and extrac- tion time is significantly reduced from 48 hours to 30 minutes. It was found that the major substances were hydrolyzable tannins, the content of which are 10% (ultrasonic extraction) and 8% (maceration), respectively. Using UV-spectrometry, it was con- firmed that the appearance of a characteristic maximum in the spectrum of the substance to be analyzed corresponds to the area of absorption of gallic acid and its derivatives, which indicates the content of these in the structure of hydrolyzable tannins. The substance shown significant antidiabetic activity (in vitro) at IC 50 = 3.94±0.14 μg/ml. Keywords: ultrasonic extraction, Tamarix hispida Willd., biologically active substances, hydrolyzable tannins, UV-spec- trometry, antidiabetic activity. * Corresponding author. У ЛЬТРАЗВУКОВОЕ ЭКСТРАГИРОВАНИЕ БИОЛОГИЧЕСКИ АКТИВНЫХ ВЕЩЕСТВ … 289 жүктеу/скачать 0,66 Mb. Достарыңызбен бөлісу:
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