Materials and methods. The plant sample, A.asiatica was collected from mountain
slopes of the Kaskasu village and air dried at room temperature. The dried material was
homogenized to obtain a coarse powder and stored in air-tight bottles. The sample preparation
includes successive extraction of plant material with non-polar and polar solvents. About 300
gm of the powdered plant raw material was subjected into successive extraction in different
extracts like chloroform, ethanol (96%) and these extracts employed in GC-MS analysis. The
Gas Chromatography-Mass Spectrometry (GC-MS) analysis of extracts of A.asiatica were
performed using a GC-MS (Model; 6890N/5973N) equipped with a DB-WAXetr column of 30
m length, diameter 0.25 µm film thickness. The column over temperature was programmed
from 40
o
C to 260
o
C for 77 min. The mass range from 34-750 m/z was scanned. 1.0 µL of the
extracts of A.asiatica was injected to the GC-MS manually for analysis. Total running time of
GC-MS is 77 min. The spectrum of the unknown component was compared with the spectrum
of the known components stored in the NIST library.
Results and discussion. The results pertaining to GC-MS analysis led to the
identification of number of compounds from the extracts of A.asiatica. These compounds were
"SCIENCE AND EDUCATION IN THE MODERN WORLD:
CHALLENGES OF THE XXI CENTURY"
NUR-SULTAN, KAZAKHSTAN, JULY 2019
354
identified through mass spectrometry attached with GC. The sample of chloroform extract was
run for 40 minutes. The spectrum profile of GC-MS confirmed the presence of 18
phytochemical components (Table 1) from A.asiatica. The phytoconstituents identified were
mainly sterols, terpenoids and alkanes (Fig. 1).
Figure 1. GC-MS Chromatogram of chloroform extract of A.asiatica
The bioactive components with their retention time (RT), CAS number and area (%) in
the chloroform extract of A.asiatica are presented in (Table 1).
S.
N
o
Retention
time
Name of the compounds
САS №
Peak Area %
1
14.387
m-Xylene
108-38-3
59362
2
14.504
p-Xylene
106-42-3
54279
3
15.058
Cumene
98-82-8
8809
4
15.352
m-Xylene
108-38-3
59362
6
18.320
4-Hydroxy-4-methyl-2-pentanone
123-42-2
175119
7
21.490
Butane acid
107-92-6
5066
8
22.019
β-Malein
489-29-2
3151
9
22.901
β-Selinen
28624-23-9
7853
1
0
23.879
α – Panasinen
56633-28-4
8024
1
1
24.878
Neophytodiene
504-96-1
42438
1
2
25.274
Octadecamethylcyclononasiloxane
556-71-8
4938081
1
3
25.558
Trans-phytol
150-86-7
25607
1
4
29.096
Hexadecanoic acid, ethyl ester
628-97-7
19610
1
5
30.148
Retinol
68-26-8
7043
1
6
30.520
Eicosomethyl cyclodexadasiloxane
18772-36-6
12386
1
7
36.938
Phytol
150-86-7
9159
1
8
39.610
Heptacosane
593-49-7
25389
Table 1. The bioactive components with their retention time
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