"фараби əлемі" атты халықаралық ғылыми конференция материалдары
THE STUDY A NUMBER ONCOGENE POLYMORPHISM OF LUNG CANCER IN THE
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thesis114326
| THE STUDY A NUMBER ONCOGENE POLYMORPHISM OF LUNG CANCER IN THE
POPULATION OF KAZAKHSTAN Abramyuk T.P. 1 , Mussa A.M. 1 , Khamdieva O.Kh. 2* 1 al-Farabi Kazakh National University 2 RSE "Institute of General Genetics and Cytology" KH MES RK azadahamdieva@gmail.com Lung cancer is the main reason for death from cancer in our country now, and further growth is predicted. In Kazakhstan, about 3,669 new cases of lung cancer are detected every year and a five-year survival period of 2014 was 33.2%. Aim of our work to study the expression of C-KIT, EGFR proteins and identify germ-line mutations in patients with lung cancer living in Almaty. Subject of the study was blood samples and biopsy material obtained from patients diagnosed with lung cancer who are being treated at the Almaty Oncology Center and residing in Almaty. All clinical data were collected, including sex, age, smoking status and clinical stage of the tumor. Polymerase chain reaction was chosen as the research methods, followed by analysis of restriction fragment length polymorphism and immunohistochemical analysis. In our studies, genetic analysis was carried out of 11 exon of C-KIT gene for the presence of mutations in codon 651. Then was performed PCR analysis with specific primers selected for exon 20 and 21 of the EGFR gene. The conducted immunohistochemical analysis has revealed that 13 (29.5%) patients had overexpression the EGFR gene, 11 (25%) patients had a moderate expression (more than 10%), 9 (20.5) patients showed a weak expression 1+ (less than 10 %) and 11 patients had no expression at all. On the whole 24 (52%) patients had a positive response to the immunohistochemical analysis. Immunohistochemical analysis performed revealed in 3 (6.82%) patients overexpression of C-KIT gene, 12 (27.27%) patients had moderate expression, 22 (50%) patients had weak expression and 7 (15.91%) patients had no expression. In total, 15 (34.09%) patients had a positive response to immunohistochemical analysis. This suggests that the disorders occurred in the cells of tumor tissue and not inherited. In fact, in most cases the occurrence of lung cancer can be associated with smoking; In addition, this neoplasm can be associated with other carcinogenic agents in a state of inhalation such as radon gas. Scientific adviser: Ph.D., Associate Professor Biyasheva Z.M. |