"фараби əлемі" атты халықаралық ғылыми конференция материалдары
THE STUDY OF THE LABORATORY MICE GERM CELLS UNDER THE ACTION OF
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| THE STUDY OF THE LABORATORY MICE GERM CELLS UNDER THE ACTION OF
UNSYMMETRIC DIMETHYLHYDRAZINE (UDMH) AND THE EXTRACT OF INULA BRITANNICA (FAMILY COMPOSITAE) Sultonova A.A. al-Farabi Kazakh National University sultonova_aliya@inbox.ru A huge number of studies are devoted to the impact of environmental factors on human reproductive health. Various factors (mutagens) were identified that can induce genetic mutations that affect the processes of cell proliferation and differentiation. In recent years, the search for and development of preparations based on plant raw materials, possessing antimutagenic and genoprotective activity, acquires particular urgency. Thereby, the purpose of this research is to study the modifying effect of Inula britannica plant extracts containing biologically active substances (BAS) on spermatogenesis in UDMH-treated laboratory mice. Quantitative analysis of the immature germ cells (QAIGC) was carried out at stages of their development in laboratory mice treated successively with UDMH at a concentration of 6.6 mg/kg and BAS complex of I.britannica at a concentration of 100 and 150 mg / kg. QAIGC is one of the most accessible methods for analyzing the state of spermatogenesis, based on determining the number of cells in different stages of spermatogenesis: pre-pachytene (leptotene and zygotene), pachytene, diplotene and metaphase. The effect of UDMH on IGC led to a block of spermatogenesis. This is evidenced by a significant decrease in the number of cells at different stages of meiotic division. Withal, the number of nuclear complexes (non-germinated spermatids) increased significantly in comparison with the control. Addition to bi- and tetranuclear complexes, multinuclear complexes were identified. The I.britannica plant extract in concentrations of 100 and 150 mg/kg had not any negative effect on the mice reproductive cells, the results obtained were not significantly different from the control. The combined effect of UDMH and plant extract statistically increased the level of dividing cells in mice comparison to the action of only UDMH. Thus, the results obtained indicate a modification of the spermatogenesis in the plant extract of I.britannica in mice exposed to UDMH. Scientific advisers: Doctor of biological science, professor Kolumbayeva S.Zh., PhD Lovinskaya A.V. |