"фараби əлемі" атты халықаралық ғылыми конференция материалдары
THE STUDY OF SOLUBILIZATION OF BROWN COAL BY ISOLATED BACTERIAL STRAINS
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| THE STUDY OF SOLUBILIZATION OF BROWN COAL BY ISOLATED BACTERIAL STRAINS
AND ITS ELEMENTAL ANALYSIS Qiao Xiaohui , Tastambek K.T. al-Farabi Kazakh National university, Almaty, Kazakhstan qiaoxiaohui1988@126.com Brown coal has attracted considerable attention as a source of energy due to the depletion of oil and natural gas reserves. However, this process generates a large number of substances that contribute to 175 environmental pollution, mainly carbon, nitrogen and sulfur oxides. Therefore, alternative, environmentally friendly technologies for the conversion of brown coal were sought. The brown coal used in this study was selected from four deposits in Kazakhstan. Five bacterial strains were isolated from the weathered lignite minerals and supported on agar slopes of Luria-Bertani (LB). The 16S rRNA gene of the five strains was amplified using individual PCR colony, with bacterial universal primers. Initial tests of lignite biosolubilization were carried out in Petri dishes. After activation 5, the bacteria were inoculated in the center of the LB agar medium in Petri dishes, and then lignite particles were distributed on the agar surface at a concentration of 1 g of lignite particles per cm 2 . Petri dishes were then transferred to a biological incubator (28°C) and observed every day. Strains of Acinetobacter pittii RKB1, Bacillus sp. RKB2, rapidly solubilized lignite samples on solid LB culture medium after 2 days. In the control experiments, there was no brown halo in the absence of inoculum or lignite. Thus, the brown halo of solubilized lignite was obtained due to the activities of Acinetobacter pittii RKB1, Bacillus sp. RKB2. Elemental analyzes of untreated lignite were carried out to measure the percentages of carbon, hydrogen and nitrogen using an elementary analyzer Vario-ELcube (China). It is planned to determine the percentage of oxygen and sulfur and processed lignite. The result was in the samples: 2-KLI N-0.47%, C- 64.68%, H-3.324%, 3-LLI N-0.48%, C-55.33%, H-2.448%, 6-OLI N-0.48%, C-60.40%, H-3.323%, 8-YLI N-0.83%, C-59.54%, H-4.121%. The next aim of the research is to study the effect on the elemental composition after microbial solubilization. Scientific adviser: Doctor of Biological Sciences, Professor Zhubanova AA жүктеу/скачать 2,79 Mb. Достарыңызбен бөлісу:
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