Objectives 1.
To study the effectiveness of various methods of using platelet-rich auto-
plasma in the area of the inter-intestinal anastomosis experimentally.
2.
To prove the mechanical strength, as well as to study the course of the
regenerative process of the formed anastomoses by injection of platelet-rich auto-
plasma in the experiment.
3.
To develop a method for preventing the failure of gastrointestinal
anastomoses using platelet-rich auto-plasma.
4.
To assess the effect of the usage of platelet-rich auto-plasma on the
healing process of the gastrointestinal anastomosis and the course of the early
postoperative period in emergency surgery.
5.
Carry out a comparative assessment of the results of treatment in groups
of patients with the use of platelet-rich auto-plasma in the area of the
gastrointestinal anastomosis and without it.
Scientific novelty of study 1.
For the first time, a method has been developed and applied in the
experiment to prevent anastomotic leak of intestinal anastomoses by using platelet-
rich auto-plasma (No. 2018/0657.1, dated September 24, 2018).
2.
Qualitatively new data on the dynamics of morphological and functional
changes in the formed anastomosis at different periods of the experiment were
obtained and the threshold levels of intestinal anastomosis failure were determined.
3.
For the first time in an experimental study, the mechanical strength of the
formed intestinal anastomoses was proved by using the proposed method of using
platelet-rich auto-plasma.
4.
New data on the effect of platelet-rich auto-plasma on the process of
adhesion formation under experimental conditions are proven.
5.
A method has been developed to improve the regeneration of
gastrointestinal anastomoses by using platelet-enriched auto-plasma (RK Patent
No. 2019/0691.1, dated 03/19/2021).
6.
A method has been developed to prevent gastrointestinal anastomotic
leaks by using an injection method for introducing platelet-rich auto-plasma in
emergency surgery (Eurasian patent (No. 2019/068, dated 18.09.2019).
7.
The nature of the course of the postoperative period with the use of
platelet-rich auto-plasma during the imposition of gastrointestinal anastomoses in
emergency surgery is shown.