T.V. Sirota, M.V. Zakharchenko, M.N. Kondrashova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences,
Institutskaya ul. 3, Pushchino, Moscow region, 142290 Russia; tel.: (7)(4967)739170;
fax: 8(4967)330553; e-mail: sirotatv@rambler.ru
Several parameters of the cytoplasmic enzymatic antioxidant system of the
liver and brain
of the rat have been investigated under conditions of immobilization
stress and of an antioxidant
preparation in the diet of animals. These included superoxide dismutase (SOD) and glutathione
reductase (GR) activities and nonspecific NADPH oxidation. Only changes in the activity of SOD
both in the liver and brain were revealed. In the liver of animals that receive no preparation,
a decrease in the activity of SOD after 30-min immobilization and its restoration after a 360-min
immobilization were observed. In the brain, the activity of SOD decreased only in preconditioned
animals after 30 and 360 min of exposure to stress. In addition, the
activity of SOD in the brain
of preconditioned animals, both stressed and unstressed, was lower than in the corresponding groups
of control animals. It is probable that, under the conditions
of immobilization stress,
the level
of reactive oxygen species (ROS) and as a consequence the activity of SOD decrease. The intake
of an antioxidant preparation under these conditions seems to be not correct.
Key words: superoxide dismutase, glutathione reductase, immobilization stress
, liver, brain.
Сирота и др.
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